Sheep leptin ELISA kit

Beschreibung

Sheep leptin ELISA kit

The kit is for the quantitative level of leptin in the sample, adopt purified Human leptin to coat microtiter plate, make
solid-phase antibody, then add leptin to wells, Combine leptin antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of leptin in the samples is then determined by comparing the O.D. of the samples
to the standard curve.