Beschreibung
The kit is for the quantitative level of TGF-β in the sample, adopt purified Rat TGF-β to coat microtiter plate, make
solid-phase antibody, then add TGF-β to wells, Combine TGF-β antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue
color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is
measured at a wavelength of 450 nm. The concentration of TGF-β in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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