Rat neutrophil gelatinase-associated lipocalin,NGAL ELISAkit

Beschreibung

Rat neutrophil gelatinase-associated lipocalin,NGAL ELISAkit

The kit is for the quantitative level of NGAL in the sample, adopt purified Human NGAL to coat microtiter plate, make
solid-phase antibody, then add NGAL to wells, Combine NGAL antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of NGAL in the samples is then determined by comparing the O.D. of the samples
to the standard curve.