Rat mast cell tryptase(MCT) ELISA Kit

Beschreibung

Rat mast cell tryptase(MCT) ELISA Kit

The kit is for the quantitative level of MCT in the sample, adopt purified Rat MCT to coat microtiter plate, make solid-phase
antibody, then add MCT to wells, Combine MCT antibody with labeled HRP to form antibody-antigen -enzyme-antibody
complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP
enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of MCT in the samples is then determined by comparing the O.D. of the samples
to the standard curve.