Rat Interleukin 6?IL-6) ELISA Kit

Beschreibung

Rat Interleukin 6?IL-6) ELISA Kit

The kit assay Rat IL-6 level in the sample, use Purified Rat IL-6 to coat microtiter plate wells, make solid-phase antibody,
then add IL-6 to wells, Combine IL-6 antibody with HRP labeled to form antibody-antigen -enzyme-antibody complex, after
washing completely, add TMB substrate solution,TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a
wavelength of 450 nm. The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples
to the standard curve.