Rat Ghrelin ELISA Kit

Beschreibung

Rat Ghrelin ELISA Kit

The kit is for the quantitative level of Ghrelin in the sample, adopt purified Rat Ghrelin to coat microtiter plate, make solid-phase antibody, then add Ghrelin to wells, Combine Ghrelin antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of Ghrelin in the samples is then determined by comparing the O.D. of the samples to the standard curve.