Rat Free Tri-iodothyronine Indes,Free-T3 ELISA Kit

Beschreibung

Rat Free Tri-iodothyronine Indes,Free-T3 ELISA Kit

The kit is for the quantitative level of Free-T3 in the sample, adopt purified Rat Free-T3 to coat microtiter plate, make
solid-phase antibody, then add Free-T3 to wells, Combine Free-T3 antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color
at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of Free-T3 in the samples is then determined by comparing the O.D. of the
samples to the standard curve.