Human basic fibroblast growth factor2 (bFGF2) ELISA Kit

Beschreibung

Human basic fibroblast growth factor2 (bFGF2) ELISA Kit

The kit is for the quantitative level of bFGF2 in the sample, adopt purified human bFGF2
antibody to coat microtiter plate, make solid-phase antibody, then add samples or
standards to wells with a labeled antibody specific to bFGF2, then add labeled HRP to
the well. After washing completely, add TMB substrate solution, TMB substrate
becomes blue color in wells that contains antibody – antigen – enzyme-antibody
complex, reaction is terminated by the addition of a stop solution and the color
change is measured at a wavelength of 450 nm. The concentration of bFGF2 in the
samples is then determined by comparing the O.D. of the samples to the standard
curve.