Beschreibung
1, 5-anhydroglucitol(1, 5-AG), Human, ELISA Kit
The kit is for the quantitative level of PYY in the sample, adopt purified Human PYY to coat microtiter plate, make
solid-phase antibody, then add PYY to wells, Combine PYY antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of PYY in the samples is then determined by comparing the O.D. of the samples
to the standard curve.
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