Rat GALECTIN-3?GALECTIN-3?ELISA Kit

Beschreibung

Rat GALECTIN-3?GALECTIN-3?ELISA Kit

The kit is for the quantitative level of Galectin-3 in the sample, adopt purified Rat Galectin-3 to coat microtiter plate,
make solid-phase antibody, then add Galectin-3 to wells, Combine Galectin-3 antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB
substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution
and the color change is measured at a wavelength of 450 nm. The concentration of Galectin-3 in the samples is
then determined by comparing the O.D. of the samples to the standard curve.